Pervaporation: Membrane Separations

G-100 is used for higher molecular mass proteins. The solutions of proteins are prepared in guanidine hydrochloride (10 mg mL ), and cytochromec (10 mg mL ) is used as an internal standard. Descending chromatography is carried out at room temperature under an inclination angle of 253 to the horizontal. After 3 h of development the quotient Rs protein/Rs cytochrome c is calculated for each protein}cytochrome c combination. A standard calibration line is obtained by plotting the log relative molecular masses of standards against protein}cytochrome quotient; the relative molecular masses of the unknown proteins are calculated from this plot.